LNA (LOCKED NUCLEIC-ACIDS) - SYNTHESIS OF THE ADENINE, CYTOSINE, GUANINE, 5-METHYLCYTOSINE, THYMINE AND URACIL BICYCLONUCLEOSIDE MONOMERS, OLIGOMERIZATION, AND UNPRECEDENTED NUCLEIC-ACID RECOGNITION

Authors
KOSHKIN AA SINGH SK NIELSEN P RAJWANSHI VK KUMAR R MELDGAARD M OLSEN CE WENGEL J
Citation
Aa. Koshkin et al., LNA (LOCKED NUCLEIC-ACIDS) - SYNTHESIS OF THE ADENINE, CYTOSINE, GUANINE, 5-METHYLCYTOSINE, THYMINE AND URACIL BICYCLONUCLEOSIDE MONOMERS, OLIGOMERIZATION, AND UNPRECEDENTED NUCLEIC-ACID RECOGNITION, Tetrahedron, 54(14), 1998, pp. 3607-3630
Citations number
30
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron → ACNP
ISSN journal
00404020
Volume
54
Issue
14
Year of publication
1998
Pages
3607 - 3630
Database
ISI
SICI code
0040-4020(1998)54:14<3607:L(N-SO>2.0.ZU;2-A
Abstract
LNA (Locked Nucleic Acids), consisting of 2'-O,4'-C-methylene bicyclon ucleoside monomers, is efficiently synthesized and its nucleic acid re cognition potential evaluated for six different nucleobases, namely ad enine, cytosine, guanine, 5-methylcytosine, thymine and uracil. Unprec edented increases (+3 to +8 degrees C per modification) in the thermal stability of duplexes towards both DNA and RNA were obtained when eva luating mixed sequences of partly or fully modified LNA. Studies of mi s-matched sequences show that LNA obey the Watson-Crick base pairing r ules with generally improved selectivities compared to the correspondi ng unmodified reference strands. (C) 1998 Elsevier Science Ltd. All ri ghts reserved.