DETERMINATION OF THIABENDAZOLE IN POTATOES BY ELISA

A method was developed to analyze thiabendazole (TBZ) in potatoes. Pot atoes were extracted in methanol for 3 min with a polytron. A 100-mu l aliquot was removed and evaporated to dryness using air. The residue was dissolved in I mi of high-performance liquid chromatography (HPLC) grade water before quantifying TBZ using an existing antibody with a tube-formatted competitive enzyme-linked immunosorbent assay (ELISA). Eight samples could be analyzed in 25 min without the use of complex a nd expensive equipment Indeed, samples could be analyzed at storage fa cilities. The detection limit for this assay in potatoes was 3 ppb. Re producibility and accuracy were good. Correlation of TBZ concentration between HPLC and ELISA was 0.9729 for 78 samples.