HEPARAN CHONDROITIN/DERMATAN SULFATE PRIMER 2-(6-HYDROXYNAPHTHYL)-O-BETA-D-XYLOPYRANOSIDE PREFERENTIALLY INHIBITS GROWTH OF TRANSFORMED-CELLS/

Xylose forms the direct carbohydrate-protein link in extra- or pericel lular proteoglycans (PGs) that are substituted with either chondroitin sulfate (CS)/dermatan sulfate (DS) and/or heparan sulfate (HS), Cell surface PGs carrying HS are important regulators of tell growth. Xylos e coupled to an aromatic compound can enter cells and initiate either CS/DS synthesis or both HS and CS/DS synthesis, depending on the natur e of the aromatic adduct. Here, we show that 2-(6-hydroxynaphthyl)-O-b eta-D-xylopyranoside, which can prime both types of glycan chains, inh ibits growth of a set of normal and transformed cells. Transformed cel ls are preferentially inhibited, and at a concentration of 0.15-0.20 m M xyloside, transformed cells are totally growth arrested, whereas nor mal cells are only less than or equal to 50% inhibited. No inhibition of growth is observed with the stereoisomeric 2-(6-hydroxynaphthyl)-O- beta-L-xylopyranoside, which does not prime glycosaminoglycan synthesi s at all; with the nonhydroxylated 2-naphthyl-O-beta-D-xylopyranoside, which only primes CS/DS synthesis under these conditions; or with p-n itrophenyl-O-beta-D-xylopyranoside, which is known to prime only CS/DS synthesis. We conclude that growth inhibition is due to priming of HS and/or CS/DS synthesis, which may either lead to the formation of spe cific antiproliferative glycans or glycan fragments or to interference with endogenous PG synthesis and turnover.