AGE-RELATED DECREASE OF NOR ACTIVITY IN BONE-MARROW METAPHASE CHROMOSOMES FROM HEALTHY-INDIVIDUALS

Aims-To present data obtained from human bone marrow preparations from healthy individuals showing that the proportion of metaphases with si lver stained nucleolar organiser region (AgNOR) chromosomes is associa ted with the age of the donor. Methods-Bone marrow preparations from e ight Russian and 10 Argentinian healthy individuals donating bone marr ow for heterologous transplantation were studied by silver staining. T he Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37 degrees C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with s light modifications. Thirty metaphases with clearly defined D and G gr oup chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed t o assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall's rank correlation coefficient (rK) were used to asse ss the relation between age and the percentage of AgNOR positive cells . Results-The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.1 7) and 5.56 (0.23) for the Russian and Argentinian groups, respectivel y, with no significant differences between groups. The common percenta ge AgNOR positive cells decreased significantly as a function of age, with an rK = -0.57 (p < 0.0012). Conclusions-The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongl y associated with age and this may be related to age related telomere loss.