RETENTION OF CANDIDA-ALBICANS ON ACRYLIC RESIN AND SILICONE OF DIFFERENT SURFACE-TOPOGRAPHY

Statement of problem. The adhesion of microorganisms to a denture surf ace is a prerequisite for colonization. Purpose. This study compared t he retention of Candida albicans on smooth and rough acrylic resin and silicone surfaces after a washing procedure to determine the effect o f surface roughness on prosthesis infection and hygiene. Material and methods. Standardized cell suspensions of C. albicans were incubated w ith smooth and rough acrylic resin and silicone surfaces for 1 hour at 24 degrees C. After washing, cells that had been retained on the surf ace were stained with acridine orange and examined with incident beam fluorescent microscopy. Results. There was no significant difference i n cell numbers on either of the smooth surfaces. Significantly higher numbers of cells (p > 0.0005) were observed on roughened surfaces (sil icone > acrylic resin) than on smooth surfaces. The fitting surface of the maxillary denture was not polished. Conclusions. Silicones used i n prostheses were processed against dental stone. The resultant surfac e roughness may facilitate microbial retention and infection and shoul d therefore be kept to a minimum.