CLONING AND CHARACTERIZATION OF MUPP1, A NOVEL PDZ DOMAIN PROTEIN

Using the yeast two-hybrid system we isolated a cDNA clone encoding a novel protein interacting with the C-terminal domain of the 5-HT2C rec eptor. The protein, named MUPP1 (multi-PDZ-domain protein), contains t hirteen PDZ domains and no obvious catalytic domain; it is related to hINADL and a putative C. elegans polypeptide referred to as C52A11.4 c ontaining six or ten PDZ domains, respectively. Domains highly similar to those of MUPP1 are arrayed in the same order in all three proteins . The MUPP1 gene is localized on human chromosome 9p24-p22. Transcript s encoding MUPP1 are abundant in the brain as well as in several perip heral organs. (C) 1998 Federation of European Biochemical Societies.