CLONING, EXPRESSION ANALYSIS AND CHROMOSOMAL LOCALIZATION OF THE HUMAN NUCLEAR RECEPTOR GENE GCNF

Germ cell nuclear factor (GCNF) is an orphan member of the nuclear rec eptor gene superfamily. We report the cloning of a cDNA encoding a nem variant of human GCNF from human testis and its expression analysis. Southern blot analysis of the human genomic DNA indicates that the GCN F gene is not closely related to other members within the nuclear rece ptor superfamily. Chromosomal localization of the GCNF shows that the gene is located on chromosome 9 at the locus q33-34.1. In situ hybridi zation analysis of GCNF expression in the testis shows that human GCNF is expressed exclusively in germ cells. (C) 1998 Federation of Europe an Biochemical Societies.