C. Yang et Dy. Zhu, GLYCOGEN ASSAY FOR DIAGNOSIS OF FEMALE GENITAL CHLAMYDIA-TRACHOMATIS INFECTION, Journal of clinical microbiology, 36(4), 1998, pp. 1081-1082
Chlamydia trachomatis can synthesize glycogen at various stages in its
developmental cycle, The glycogen content of female genital epithelia
l cells was detected by anthrone, and the results were compared with t
hose from PCR, A total of 320 cervical samples were examined. Of 92 sp
ecimens that were positive by PCR, 78 were positive and 14 were negati
ve by the glycogen assay, Of 228 specimens that were negative by PCR,
220 were negative and 8 were positive by the glycogen assay, The sensi
tivity and specificity of the glycogen assay obtained from these data
were 84.8% (78 of 92) and 96.5% (220 of 228), respectively. Use of the
glycogen assay to detect the glycogen content in genital epithelial c
ells may be helpful in the diagnosis of C. trachomatis infection, This
is an easy, fast, and inexpensive assay and can be done in Less-sophi
sticated labs.