DEVELOPMENT OF A MONOCLONAL-ANTIBODY TO A UREAPLASMA-UREALYTICUM SEROTYPE-9 ANTIGEN

We produced a monoclonal antibody (MAb) to Ureaplasma urealyticum Vanc ouver, the serotype 9 standard strain. By immunoblotting, this MAb sho wed a single, 85-kDa band with the homologous serotype and a minor, 10 0-kDa band with serotype 2 but did not react with any other serotype s tandard strain. Clinical isolates of U. urealyticum were tested with t his MAb and with two sets of polyclonal antisera against the 14 seroty pe standard strains. The use of MAb 9-2H9 correctly identified certain serotype 9 strains but did not react with wild-type strains lacking t he serotype 9 determinant.