BILE-ESCULIN TEST FOR PRESUMPTIVE IDENTIFICATION OF ENTEROCOCCI AND STREPTOCOCCI - EFFECTS OF BILE CONCENTRATION, INOCULATION TECHNIQUE, AND INCUBATION-TIME

Authors
CHUARD C RELLER LB
Citation
C. Chuard et Lb. Reller, BILE-ESCULIN TEST FOR PRESUMPTIVE IDENTIFICATION OF ENTEROCOCCI AND STREPTOCOCCI - EFFECTS OF BILE CONCENTRATION, INOCULATION TECHNIQUE, AND INCUBATION-TIME, Journal of clinical microbiology, 36(4), 1998, pp. 1135-1136
Citations number
13
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
4
Year of publication
1998
Pages
1135 - 1136
Database
ISI
SICI code
0095-1137(1998)36:4<1135:BTFPIO>2.0.ZU;2-Y
Abstract
The bile-esculin test is used to differentiate enterococci and group D streptococci from non-group D viridans group streptococci. The effect s on test performance of the concentration of bile salts, inoculum, an d duration of incubation were examined with 110 strains of enterococci , 30 strains of Streptococcus bovis, and 110 strains of non-group D vi ridans group streptococci. Optimal sensitivity (>99%) and specificity (97%) of the bile-esculin test can be obtained with a bile concentrati on of 40%, a standardized inoculum of 10(6) CFU, and incubation for 24 h.