C. Vanderstruijf et Yk. Levine, ON THE USE OF THE PHASE MEMORY TIME T-2 FOR THE QUANTITATIVE CHARACTERIZATION ION OF THE ROTATIONAL MOTIONS OF PROTEINS IN LIPID BILAYER SYSTEMS, Journal of magnetic resonance [1997], 130(2), 1998, pp. 244-252
Citations number
23
Categorie Soggetti
Physics, Atomic, Molecular & Chemical","Biochemical Research Methods
Numerical simulations of the echo responses from a nitroxide label rig
idly attached to a large protein undergoing ultraslow rotational motio
ns in a lipid bilayer are presented, The echoes are formed by the appl
ication of Hahn, COSY, and 2D-ELDOR sequences utilizing both soft and
hard microwave pulses. The simulations address the question of whether
the echo responses elicited by these sequences are affected by restri
cted angular excursions of the long axis of the protein relative to th
e normal to the bilayer plane, The results indicate that all three pul
se sequences yield the same quantitative motional information regardle
ss of the nature of the microwave pulses and there is no theoretical r
eason for preferring one sequence above the others. (C) 1988 Academic
Press.