QUANTITATIVE INVESTIGATION OF DIPOLE-CSA CROSS-CORRELATED RELAXATION BY ZQ DQ SPECTROSCOPY/

Authors
BRUTSCHER B SKRYNNIKOV NR BREMI T BRUSCHWEILER R ERNST RR
Citation
B. Brutscher et al., QUANTITATIVE INVESTIGATION OF DIPOLE-CSA CROSS-CORRELATED RELAXATION BY ZQ DQ SPECTROSCOPY/, Journal of magnetic resonance [1997], 130(2), 1998, pp. 346-351
Citations number
24
Categorie Soggetti
Physics, Atomic, Molecular & Chemical","Biochemical Research Methods
Journal title
Journal of magnetic resonance [1997] → ACNP
Volume
130
Issue
2
Year of publication
1998
Pages
346 - 351
Database
ISI
SICI code
Abstract
A zero-quantum/double-quantum HNCO(H) constant time experiment is pres ented for the quantitative evaluation of dipole-CSA cross-correlated r elaxation involving the H-1(N), N-15, and C-13' nuclei of the peptide plane. A simple procedure that allows the extraction of cross-correlat ed relaxation rate constants from intensity ratios of well-resolved do ublet components along omega(1) is described. The experiment is demons trated on fully C-13, N-15-labeled ubiquitin. (C) 1998 Academic Press.