STUDIES OF SOME CHARACTERS OF MATRIX INFLUENCING DESORPTION IONIZATION PROCESSES IN MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY

Citation
Sk. Zhao et al., STUDIES OF SOME CHARACTERS OF MATRIX INFLUENCING DESORPTION IONIZATION PROCESSES IN MATRIX-ASSISTED LASER-DESORPTION IONIZATION MASS-SPECTROMETRY, Analytical sciences, 13, 1997, pp. 45-48
Citations number
22
Journal title
ISSN journal
09106340
Volume
13
Year of publication
1997
Supplement
S
Pages
45 - 48
Database
ISI
SICI code
0910-6340(1997)13:<45:SOSCOM>2.0.ZU;2-0
Abstract
Some results are introduced in studying characters of matrices influen cing the desorption ionization processes in MALDI-MS, which have not b een reported. By the use of low angle laser scattering photometer the association state of four matrices with lysozyme are measured. The res ults demonstrated that 3-amino-4-hydroxybenzoic acid (3A4HA) is the be st one which call be calculated 224 molecules to associate with one mo lecule of lysozyme. The other three matrices, nicotinic acid (NA), 2,5 dihydroxybenzoic acid (DHB) and caffeic acid (CA) are in the order of several tens of matrix molecules associated with one protein molecule . It is interesting to point out that 3-amino-4-hydroxybenzoic acid is the only one that cannot perform MALDI-MS spectrum and the reason is discussed. The results of the investigation in negative MALDI mass spe ctra of different matrices demonstrated that the desorption ionization processes are different with that of positive ions, and their mechani sms were proposed.