DETERMINATION OF TRANEXAMIC ACID AND VITAMINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH PEROXYOXALATE CHEMILUMINESCENCE DETECTION

A high performance liquid chromatographic determination of tranexamic acid and two vitamins, i.e., vitamin U chloride and anthranilic acid, with chemiluminescence detection was investigated. o-Phthalaldehyde(OP A) solution which contained 2-mercaptoethanol was used as a fluorescen t labeling reagent. OPA solution was prepared by mixing OPA and 2-merc aptoethanol in ethanol, and then diluting with berate buffer solution. Bis(2,4,6-trichlorophenyl)oxalate and hydrogen peroxide were used a p ostcolumn chemiluminescent reagent. The intensity of the chemiluminesc ence was in the order of tranexamic acid> vitamin U chloride>anthranil ic acid. The calibration curves for the compounds were linear at least in the range from 1 x 10(-7)M to 1 x 10(-4)M. The detection limits fo r tranexamic acid, vitamin U chloride and anthranilic acid were 5 x 10 (-8)M, 4 x 10(-7)M and 7 x 10(-7)M, respectively. The relative standar d deviation was less than 9 %(n=5). The proposed method was applied to the determination of tranexamic acid and vitamin U chloride in commer cially available medicine.