F-ACTIN IDENTIFICATION USING PHALLOIDIN COLLOIDAL GOLD - 2 - CELLS AND SUBCELLULAR PREPARATIONS

Structures containing filament actin (F-actin) were revealed on ultrat hin sections and in subcellular preparations from the root tips of whe at (Triticum aestivum L., cv. Saratovskaya 29) seedlings and stem apic al meristems of Chenopodium rubrum using a synthesized phalloidin-coll oidal gold marker. Several types of F-actin-containing structures were found in these plant tissues: thick filament bundles crossing the cel l; a cytoplasmic microfilament network enclosing, in particular, intra cellular organelles; a subcortical network of fine filaments; and intr avesicular F-actin-containing structures.