IDENTIFICATION OF THE T-COMPLEX-ENCODED CYTOPLASMIC DYNEIN LIGHT-CHAIN TCTEX1 IN INNER ARM-I1 SUPPORTS THE INVOLVEMENT OF FLAGELLAR DYNEINSIN MEIOTIC DRIVE
A. Harrison et al., IDENTIFICATION OF THE T-COMPLEX-ENCODED CYTOPLASMIC DYNEIN LIGHT-CHAIN TCTEX1 IN INNER ARM-I1 SUPPORTS THE INVOLVEMENT OF FLAGELLAR DYNEINSIN MEIOTIC DRIVE, The Journal of cell biology, 140(5), 1998, pp. 1137-1147
The cytoplasmic dynein light chain Tctex1 is a candidate for one of th
e distorter products involved in the non-Mendelian transmission of mou
se t haplotypes. It has been unclear, however, how the t-specific muta
tions in this protein, which is found associated with cytoplasmic dyne
in in many tissues, could result in a male germ cell-specific phenotyp
e. Here, we demonstrate that Tctex1 is not only a cytoplasmic dynein c
omponent, but is also present both in mouse sperm and Chlamydomonas fl
agella. Genetic and biochemical dissection of the Chlamydomonas flagel
lum reveal that Tctex1 is a previously undescribed component of inner
dynein arm Il. Combined with the recent identification of another puta
tive t complex distorter, Tctex2, within the outer dynein arm, these r
esults support the hypothesis that transmission ratio distortion (meio
tic drive) of mouse t haplotypes involves dysfunction of both flagella
r inner and outer dynein arms but does not require the cytoplasmic iso
zyme.