GENE-TRANSFER TO RODENT BRAIN WITH RECOMBINANT ADENOVIRAL VECTORS - EFFECTS OF INFUSION PARAMETERS, INFECTIOUS TITER, AND VIRUS CONCENTRATION ON TRANSDUCTION VOLUME

Initial studies examining intraparenchymal injection of recombinant vi ral vectors in rodent brain have demonstrated a limited region of gene transfer. We examined, independently, different infusion parameters t o determine if the volume of cells transduced acutely could be increas ed. Varying the rate of infusion from 0.3 to 3 mu l/min at constant ti me and virus dose did not improve the volume of brain transduced, with the lowest rate of infusion demonstrating the least amount of gene tr ansfer. However, transduction volume did increase with increasing part icle concentrations of virus, although the improvements were modest fr om 3 to 9 x 10(9) total particles infused. Infusion of virus under con ditions of hypertonicity resulted in modest improvements in the final transduced volume. These studies suggest that most changes in infusion parameters will have small effects on the initial transduction volume in rodent brain. (C) 1998 Academic Press.