AFFINITY PURIFICATION OF RECOMBINANT TRYPSINOGEN USING IMMOBILIZED ECOTIN

Authors
LENGYEL Z PAL G SAHINTOTH M
Citation
Z. Lengyel et al., AFFINITY PURIFICATION OF RECOMBINANT TRYPSINOGEN USING IMMOBILIZED ECOTIN, Protein expression and purification, 12(2), 1998, pp. 291-294
Citations number
5
Categorie Soggetti
Biochemical Research Methods",Biology,"Biothechnology & Applied Migrobiology
Journal title
Protein expression and purification → ACNP
ISSN journal
10465928
Volume
12
Issue
2
Year of publication
1998
Pages
291 - 294
Database
ISI
SICI code
1046-5928(1998)12:2<291:APORTU>2.0.ZU;2-4
Abstract
Affinity purification of inactive precursors (zymogens) of serine prot eases on protease inhibitor columns is not feasible, due to the weak i nteraction between canonical protease inhibitors and protease zymogens . In this study we demonstrate that immobilized ecotin, a unique prote ase inhibitor from Escherichia coli, provides a superior affinity matr ix for the purification of trypsinogen and possibly other serine prote ase zymogens as well. (C) 1998 Academic Press.