Z. Lengyel et al., AFFINITY PURIFICATION OF RECOMBINANT TRYPSINOGEN USING IMMOBILIZED ECOTIN, Protein expression and purification, 12(2), 1998, pp. 291-294
Citations number
5
Categorie Soggetti
Biochemical Research Methods",Biology,"Biothechnology & Applied Migrobiology
Affinity purification of inactive precursors (zymogens) of serine prot
eases on protease inhibitor columns is not feasible, due to the weak i
nteraction between canonical protease inhibitors and protease zymogens
. In this study we demonstrate that immobilized ecotin, a unique prote
ase inhibitor from Escherichia coli, provides a superior affinity matr
ix for the purification of trypsinogen and possibly other serine prote
ase zymogens as well. (C) 1998 Academic Press.