DETECTION OF GOOSE AND MUSCOVY DUCK PARVOVIRUSES USING POLYMERASE CHAIN REACTION-RESTRICTION ENZYME FRAGMENT LENGTH POLYMORPHISM ANALYSIS

By using primers (AL18F2 and AL18R2) designed from goose parvovirus (G PV) strain IHC, an 806-bp band was amplified by polymerase chain react ion (PCR) from all of 17 samples from Thailand. Specificity to GPV was confirmed by Southern hybridization. With restriction enzyme digestio n of the PCR products, two isolates differed from the other 15 isolate s by the absence of restriction sites for HincII and BglII and the pre sence of EcoRI site. Nucleotide sequence analysis of the PCR products from the different groups revealed that one group is GPV and the other group is Muscovy duck parvovirus (MDPV). Thus restriction enzyme frag ment length polymorphism analysis of the PCR products could be used to distinguish GPV and MDPV. The data showed that GPV and MDPV are prese nt in Thailand.