FURTHER CHARACTERIZATION OF HUMAN RNASE MRP RNASE-P AND RELATED AUTOANTIBODIES

We characterized a panel of human RNase MRP/RNase P autoantibodies by immunoprecipitation, immunodepletion, immunoaffinity purification and immunoblotting. We report on the protein spectrum that is recognized b y RNase MRP/RNase P autoantibodies. We also describe another, related patient serum that based on these assays does not immunoprecipitate RN aseP/MRP/Th40. This autoantibody 'KC', however, coimmunoprecipitates t he RNase MRP/RNase P associated RNAs from HeLa and La9 cell extracts a s shown by nuclease protection experiments.