INTESTINAL MUCOSAL LIPID-PEROXIDATION AND ABSORPTIVE FUNCTION IN SALMONELLA-TYPHIMURIUM MEDIATED INTESTINAL INFECTION

S, typhimurium infection is associated with neutrophil infiltration wi thin the intestinal mucosa. Neutrophil activation provides a major sou rce of reactive oxygen species (ROS). The mucosal pathology of S. typh imurium infection may be in part due to the excessive production of th ese reactive species. This study was carried out to investigate if ROS play a role in mediating the changes in the structural components and functional properties of brush border membrane (BBM) in rats during S . typhimurium infection, This was done by determining the changes in t he BBM extent of lipid peroxidation and absorptive function. A signifi cant increase in the extent of lipid peroxidation of BBM during S. typ himurium infection was observed as judged by malondialdehyde (MDA) and conjugated diene formation and depletion of a-tocopherol and protein associated thiol groups. A significant decrease in the BBMV (brush bor der membrane vesicle) transport of amino acids was also observed. Howe ver there was no change in the transport of D-glucose. The decrease in amino acid transport further led to a significant decrease in the ent erocyte level of protein synthesis. Exposure of BBMV to a free radical donor, cumene hydroperoxide, also led to an increase in the extent of lipid peroxidation and a decrease in the amino acid transport. Possib ly ROS might play a significant role in mediating the mucosal damage d uring S. typhimurium infection.