T. Iantomasi et al., GLUTATHIONE INVOLVEMENT ON THE INTESTINAL NA-DEPENDENT D-GLUCOSE ACTIVE TRANSPORTER(), Molecular and cellular biochemistry, 178(1-2), 1998, pp. 387-392
Glutathione and its related enzymes are present in intestinal epitheli
um. Depletion or alteration of glutathione levels have been related to
different physiological and pathological conditions. Glutathione also
seems to be related to the regulation of some protein activities. The
present study, by in vivo experiments, shows a specific relationship
between D-glucose Na+-dependent active transporter activity in rat int
estine brush-border membranes and reduced glutathione/oxidized glutath
ione ratio levels. Changes of the kinetic parameters show that an incr
ease of this ratio is related to an increase of the affinity of glucos
e for its binding sites and a higher transport capacity of the transpo
rter. Neither alteration in the activity of other substrate transport
systems nor change in the specific activity of the key enzymes related
to glutathione and glucose metabolism are found. These findings sugge
st the possibility that D-glucose transporter activity is modulated th
rough the change in the redox status of glutathione.