PHOSPHOPEPTIDES DERIVED FROM IN-VITRO PHOSPHORYLATED ESCHERICHIA-COLIRNA-POLYMERASE BIND TO DNA AND AFFECT DNA-TRANSCRIPTION

Authors
CARDELLINI E PICCININI G GIANFRANCESCHI GL
Citation
E. Cardellini et al., PHOSPHOPEPTIDES DERIVED FROM IN-VITRO PHOSPHORYLATED ESCHERICHIA-COLIRNA-POLYMERASE BIND TO DNA AND AFFECT DNA-TRANSCRIPTION, Molecular and cellular biochemistry, 178(1-2), 1998, pp. 393-396
Citations number
8
Categorie Soggetti
Biology,"Cell Biology
Journal title
Molecular and cellular biochemistry → ACNP
ISSN journal
03008177
Volume
178
Issue
1-2
Year of publication
1998
Pages
393 - 396
Database
ISI
SICI code
0300-8177(1998)178:1-2<393:PDFIPE>2.0.ZU;2-B
Abstract
E. Coli RNA polymerase was phosphorylated with protein kinase CKII and allowed to bind to pBR322. After digestion of the RNA polymerase-pBR3 22 complex with proteinase K, the phosphopeptides that remained bound to DNA were extracted and analyzed. These phosphopeptides are able to bind again to DNA and to inhibit transcription.