CONVENIENT APPROACHES TO THE SYNTHESIS OF OLIGONUCLEOTIDE MACROCYCLESCONTAINING NON-NUCLEOTIDE LINKERS

Two convenient, practical routes to the synthesis of non-nucleotide br idged cyclic oligonucleotides have been developed. The first procedure included circularization of oligonucleotides by template-directed lig ation on solid phase, while the second procedure involved preparation of a circular oligomer by non-template chemical ligation of a linear p recursor in solution. Using these approaches, a series of single- and double-stranded cyclic oligonucleotides with non-nucleotide bridges ha s been synthesized.