Ql. Li et Dt. Canvin, ENERGY-SOURCES FOR HCO3- AND CO2 TRANSPORT IN AIR-GROWN CELLS OF SYNECHOCOCCUS UTEX-625, Plant physiology, 116(3), 1998, pp. 1125-1132
Light-dependent inorganic C (C-i) transport and accumulation in air-gr
own cells of Synechococcus UTEX 625 were examined with a mass spectrom
eter in the presence of inhibitors or artificial electron accepters of
photosynthesis in an attempt to drive CO2 or HCO3- uptake separately
by the cyclic or linear electron transport chains. In the presence of
3-(3,4-dithlorophenyl)-1,1-dimethylurea, the cells were able to accumu
late an intracellular C-i pool of 20 mM, even though CO2 fixation was
completely inhibited, indicating that cyclic electron flow was involve
d in the C-i-concentrating methanism. When 200 mu M N,N-dimethyl-p-nit
rosoaniline was used to drain electrons from ferredoxin, a similar C-i
accumulation was observed, suggesting that linear electron flow could
support the transport of C-i. When carbonic anhydrase was not present
, initial CO2 uptake was greatly reduced and the extracellular [CO2] e
ventually increased to a level higher than equilibrium, strongly sugge
sting that CO2 transport was inhibited and that C-i accumulation was t
he result of active HCO3- transport. With 3-(3,4-dichlorophenyl)-1,1-d
imethylurea-treated cells, C-i transport and accumulation were inhibit
ed by inhibitors of CO2 transport, such as COS and Na2S, whereas Li+,
an HCO,(-)(3)-transport inhibitor, had little effect. In the presence
of N,N-dimethyl-p-nitrosoaniline, C-i transport and accumulation were
not inhibited by COS and Na2S but were inhibited by Li+. These results
suggest that CO2 transport is supported by cyclic electron transport
and that HCO3- transport is supported by linear electron transport.