MOLECULAR-PROPERTIES OF THE ERYTHROMYCIN RESISTANCE PLASMID PPV141 FROM STAPHYLOCOCCUS CHROMOGENES

Authors
SOMKUTI GA SOLAIMAN DKY STEINBERG DF
Citation
Ga. Somkuti et al., MOLECULAR-PROPERTIES OF THE ERYTHROMYCIN RESISTANCE PLASMID PPV141 FROM STAPHYLOCOCCUS CHROMOGENES, Plasmid, 37(2), 1997, pp. 119-127
Citations number
34
Categorie Soggetti
Genetics & Heredity
Journal title
Plasmid → ACNP
ISSN journal
0147619X
Volume
37
Issue
2
Year of publication
1997
Pages
119 - 127
Database
ISI
SICI code
0147-619X(1997)37:2<119:MOTERP>2.0.ZU;2-9
Abstract
The 2.3-kb erythromycin resistance (Em(R)) plasmid pPV141 of Staphyloc occus chromogenes 3688 was isolated and characterized. Nucleotide sequ ence analysis identified ORF1 and ORF2 separated by a 445-bp spacing, encoding a 158-residue replication protein (Rep141) and a 244-residue erythromycin resistance protein (Erm, rRNA adenine N-6-methyltransfera se), respectively. Structural analysis and Southern hybridization show ed that the rep and ermM genes in pPV141 shared homology with other kn own Em(R) plasmids. Based on sequence analysis, pPV141 was classified as a unique member of the pSN2 family of Em(R) plasmids. (C) 1997 Acad emic Press.