C. Keshava et al., PROTECTIVE EFFECT OF VANILLIN ON RADIATION-INDUCED MICRONUCLEI AND CHROMOSOMAL-ABERRATIONS IN V79 CELLS, Mutation research. Fundamental and molecular mechanisms of mutagenesis, 397(2), 1998, pp. 149-159
Vanillin (VA), an anticlastogen, has been demonstrated to inhibit gene
mutations in both bacterial and mammalian cells. However, the data on
its effect against radiation-induced cytogenetic damage are limited.
The aim of this study was to investigate the protective effect of VA o
n radiation-induced chromosomal damage in V79 cells. Exponentially gro
wing cells were exposed to five doses of X-rays (1-12 Gy) and UV radia
tion (50-800 mu J x 10(2) cm(-2)) and posttreated with 3 concentration
s of VA (5, 50 or 100 mu g ml(-1)) for 16 h for micronucleus(MN)and 18
h for structural chromosomal aberration (SCA) analyses. MN and SCA as
says were performed concurrently according to standard procedures. Res
ults indicate that there was a dose related increase in the percent of
micronucleated binucleated cells (MNBN) (5.6 to 79.6) and percent of
aberrant cells (Abs) (12 to 98) with X-ray treatment alone, Inhibition
studies showed that the addition of VA at 100 mu g ml(-1) significant
ly reduced the percent of MNBN (21 to 48) induced by X-rays at 1, 2, a
nd 4 Gy. There was a slight decrease in percent MNBN at 5 and 50 mu g
VA ml(-1). All three concentrations of VA decreased percent Abs (15.7
to 57.1) induced by X-rays at all doses. UV radiation alone significan
tly increased percent MNBN (3.5 to 14.8) and percent Abs (17 to 29). A
ddition of 50 or 100 mu g VA ml(-1), significantly decreased percent M
NBN (31.7 to 86.2) and percent Abs (54.5 to 90.9) at all doses of UV r
adiation. A decrease in percent MNBN (2.8 to 72.4) and percent Abs (34
.8 to 66.7) was also noted at 5 mu g VA ml(-1). These data clearly ind
icate the protective effect of VA on radiation-induced chromosomal dam
age,suggesting that VA is an anticlastogenic agent. (C) 1998 Elsevier
Science B.V.