DEVELOPMENT OF A SCINTILLATION PROXIMITY ASSAY FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-GAMMA LIGAND-BINDING DOMAIN

Authors
NICHOLS JS PARKS DJ GONSLER TG BLANCHARD SG
Citation
Js. Nichols et al., DEVELOPMENT OF A SCINTILLATION PROXIMITY ASSAY FOR PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR-GAMMA LIGAND-BINDING DOMAIN, Analytical biochemistry, 257(2), 1998, pp. 112-119
Citations number
19
Categorie Soggetti
Biology,"Biochemical Research Methods","Chemistry Analytical
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
257
Issue
2
Year of publication
1998
Pages
112 - 119
Database
ISI
SICI code
0003-2697(1998)257:2<112:DOASPA>2.0.ZU;2-Y
Abstract
A scintillation proximity assay for peroxisome proliferator-activated receptor gamma ligand binding domain is described, Scintillation proxi mity offers an equilibrium method for detecting ligands that is both c ost effective and fully automatable. The method described here is the first reported scintillation proximity assay for a peroxisome prolifer ator-activated receptor. The design of this system is generic in natur e, allowing it to be adapted for other ligand binding proteins. (C) 19 98 Academic Press.