LONG-TERM INTRACRANIAL CANNULA STABILIZATION IN MICE WITH LIGHT-CUREDRESIN COMPOSITES

A simple and rapid method is described for the introduction and stabil ization of chronic indwelling cannulae in mice. Materials and adhesive bonding techniques commonly used in restorative dentistry were used t o stabilize the cannulae. The effectiveness of this method was evaluat ed by shear strength testing across time and histological evaluation o f coronal brain sections. Shear strength increased during the first we ek post surgery and remained stable for up to 60 days. The force requi red to dislodge the cannulae from skulls ranged from 0.70 to 2.45 kg. Histological evaluation revealed no significant infection or inflammat ory response due to the materials used. Cannulae stabilization by this technique appears to be suitable for most experimental conditions. (C ) 1998 Elsevier Science B.V.