SIMULTANEOUS FLUOROMETRIC-DETERMINATION OF PYRIDOXAL, PYRIDOXAMINE AND PYRIDOXIC ACID BY PARTIAL LEAST-SQUARES USING NONLINEAR VARIABLE-ANGLE SYNCHRONOUS SPECTRA

Authors
NEVADO JJB PULGARIN JAM AMADORHERNANDEZ J LAGUNA MAG
Citation
Jjb. Nevado et al., SIMULTANEOUS FLUOROMETRIC-DETERMINATION OF PYRIDOXAL, PYRIDOXAMINE AND PYRIDOXIC ACID BY PARTIAL LEAST-SQUARES USING NONLINEAR VARIABLE-ANGLE SYNCHRONOUS SPECTRA, Analyst, 123(3), 1998, pp. 483-488
Citations number
27
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst → ACNP
ISSN journal
00032654
Volume
123
Issue
3
Year of publication
1998
Pages
483 - 488
Database
ISI
SICI code
0003-2654(1998)123:3<483:SFOPPA>2.0.ZU;2-5
Abstract
The multivariate calibration method partial least squares regression t ype 1 (PLS-1) was applied to the simultaneous fluorimetric determinati on of pyridoxal, pyridoxamine and pyridoxic acid at lo tv concentratio n levels (8.0-80, 30.0-300 and 2.4-24 mu g l(-1), respectively), The n on-linear variable-angle synchronous spectra were obtained, tracing a curved trajectory through the total luminescence spectroscopic data, S everal calibration models were studied in order to select the best one , The statistical parameters obtained by internal and external validat ion of the optimum calibration model were in agreement, with satisfact ory results.