MIMETIC LIGAND-BASED AFFINITY PURIFICATION OF IMMUNE-COMPLEXES AND IMMUNOCONJUGATES

We developed a simple purification method to purify alkaline phosphata se/anti-alkaline phosphatase IgG as immune complexes using mimetic aff inity chromatography wherein the antibody was either a monospecific an tibody, a bispecific antibody or a commercial polyclonal IgG conjugate d with alkaline phosphatase (AP-IgG) covalently. The immune complexes or conjugates were efficiently bound on the mimetic Blue A6XL column a nd eluted under mild conditions (5-20 mM phosphate buffer). A similar strategy of purifying peroxidase/anti-peroxidase antibody complexes wa s also successfully demonstrated using the mimetic Red 3 column. Mimet ic affinity chromatography thus appears to be a simple method to purif y the desired monospecific or bispecific antibodies from the respectiv e hybridomas and quadromas. (C) 1998 Elsevier Science B.V.