SHORT COLUMN GAS-CHROMATOGRAPHY MASS-SPECTROMETRY AND PRINCIPAL COMPONENT ANALYSIS FOR THE IDENTIFICATION OF COELUTED SUBSTANCES IN DOPING CONTROL ANALYSIS

The identification of four doping control substances in an artificial mixture, using short column gas chromatography-mass spectrometry (CC-M S) analysis was examined. Two chromatographic peaks were recorded in t he chromatogram, using a short capillary column (1.8 m) at an oven tem perature of 180 degrees C. The first peak was associated with a mixtur e of a solvent derivative and an artifact. The second one corresponded to the mixture of four control substances. Principal component analys is was applied on a selected CC-Ms data set of the latter peak to dete rmine clear full spectra of pure substances from mixture spectra. The time of GC-MS analysis was significantly reduced to less than 1 min fr om 30 min which is a typical GC-MS analysis time, using standard metho ds of doping control analysis. (C) 1998 Elsevier Science B.V.