QUANTIFICATION OF PYRAZINAMIDE AND ITS METABOLITES IN PLASMA BY IONIC-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - IMPLICATIONS FOR THE SEPARATION MECHANISM
Hj. Kraemer et al., QUANTIFICATION OF PYRAZINAMIDE AND ITS METABOLITES IN PLASMA BY IONIC-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - IMPLICATIONS FOR THE SEPARATION MECHANISM, Journal of chromatography B. Biomedical sciences and applications, 706(2), 1998, pp. 319-328
Citations number
21
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
Pyrazinamide, the amide of pyrazinoic acid, is one of the basic therap
eutic agents currently used in combination for chemotherapy of tubercu
losis. A reversed-phase high-performance liquid chromatography method
based on ionic pair chromatography, was developed after solid-phase ex
traction of the analytes from plasma with prior addition of internal s
tandard. The main metabolites, pyrazinoic acid, 5-hydroxypyrazinoic ac
id and 5-hydroxypyrazinamide, were included as well as uric acid and o
ther purine derivatives to allow detailed study of the pharmacokinetic
s of the drug, especially in patients with impaired kidney function. S
ome interesting features of the chromatographic system giving some ins
ight in the retention mechanism and of the solid-phase extraction are
discussed in detail. (C) 1998 Elsevier Science B.V.