ANALYSIS OF MINOCYCLINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN TISSUE AND SERUM

A sensitive and rapid reversed-phase high-performance liquid chromatog raphy assay can be used to accurately determine serum and tissue minoc ycline concentrations. Minocycline is a broad spectrum tetracycline de rivative with many applications. Tissue and serum samples were obtaine d from guinea pigs that had received either topical or intravenous min ocycline. Samples were extracted using a Sep-Pak C-18 cartridge and we re injected into a mu Bondapak C-18 column with an isocratic methanol mobile phase. Samples were analyzed using UV detection and produced sh arp peaks with a retention time of 2.5 min. The lower limit of detecti on was 100 ng and drug recovery was 61%. This method greatly facilitat ed the analysis of minocycline while allowing for sensitivity. (C) 199 8 Elsevier Science B.V.