22-MB INTEGRATED PHYSICAL AND GENETIC-MAP BASED ON YAC STS CONTENT SPANNING THE INTERVAL DXS1125-DXS95 IN HUMAN XQ12-Q21.31/

A YAC/STS map has been assembled spanning 22 Mb across Xq12-q21.31, be tween markers DXS1125 and DXS95. Ln addition to the landmark loci for the X-inactivation center XIST and the ATRX, ATP7A, phosphoglycerate k inase, POU3F4, and choroideremia genes, the candidate disease gene reg ions for torsion dystonia 3 and two X-linked mental retardation syndro mes are included. Also, the human voltage-dependent anion channel gene (HVDAC1) has been placed near DXS986. The current map incorporates 21 1 YACs from five different libraries, formatted with 185 STSs that com prise 26 genetic linkage markers, 60 newly-developed YAC-end STSs, and eight ESTs. The multiple clone coverage and average resolution of one STS per 120 kb provide resources for disease gene searches and are fa cilitating complete sequencing of the region. (C) 1998 Elsevier Scienc e B.V.