TECHNIQUE FOR PERFORMING DIRECT LYMPHANGIOGRAPHY IN PIGS

Rationale and Objectives. The authors developed a porcine model for di rect lymphangiography to be used as a reference for lymph node harvest ing. Methods. A pilot animal was studied first to develop the protocol , which was used successfully in 24 pigs. An indicator dye was first i njected into each foot to make the lymphatic vessels visible. Then, et hiodized poppy seed oil was injected directly through cutdowns in both groins. Radiographs were obtained before and after lymph node harvest ing. Results. Images were of diagnostic quality in all animals. The pi lot animal developed transient respiratory distress, which was thought to be due to the oil-based contrast material. The amount injected was reduced by half, and no episodes occurred thereafter in the study gro up. There were no other immediate or delayed complications. Conclusion . Direct lymphangiography is safe, easy, and reliable in a porcine mod el.