APPLICATION OF A LARGE ASSEMBLY OF MICROELECTRODES TO THE STUDY OF CYTOCHROME-C AND OTHER REDOX PROTEINS

A novel microelectrode assembly containing more than 1000 carbon micro disks has been used to study the interfacial electron transfer kinetic s of cytochrome c and several other redox proteins. It is demonstrated that near-steady-state current-voltage curves can be obtained without solution stirring, and without significant mains interference or capa citive charging currents. In addition, it is demonstrated that concent rations of redox proteins as low as 1 mu M can be detected in solution . Finally, it is shown that the surfaces of carbon microdisk assemblie s can be successfully coated with thin films of poly(ester sulfonic ac id), which allows reactants to be studied in a membrane-like environme nt.