P. Bianco et al., APPLICATION OF A LARGE ASSEMBLY OF MICROELECTRODES TO THE STUDY OF CYTOCHROME-C AND OTHER REDOX PROTEINS, Electroanalysis, 9(4), 1997, pp. 307-310
A novel microelectrode assembly containing more than 1000 carbon micro
disks has been used to study the interfacial electron transfer kinetic
s of cytochrome c and several other redox proteins. It is demonstrated
that near-steady-state current-voltage curves can be obtained without
solution stirring, and without significant mains interference or capa
citive charging currents. In addition, it is demonstrated that concent
rations of redox proteins as low as 1 mu M can be detected in solution
. Finally, it is shown that the surfaces of carbon microdisk assemblie
s can be successfully coated with thin films of poly(ester sulfonic ac
id), which allows reactants to be studied in a membrane-like environme
nt.