EFFICIENT SELECTION OF CELLS WITH HIGH TA XOL CONTENT FROM HETEROGENEOUS TAXUS CELL-SUSPENSIONS BY MAGNETIC OR FLUORESCENT-ANTIBODIES

Small cell clusters with high taxol contents were selected in the foll owing manner. Cell suspensions derived from Taxus brevifolia and Taxus cuspidata were reacted with primary polyclonal antibodies of taxol, a nd then labeled by magnetic or fluorescent (FITC) secondary antibodies . Cell clusters bound to the secondary antibodies were picked up manua lly by a small magnet or through a fluorescence microscope. It was pos sible to seperate cell clusters having taxol contents of more than 100 0 mu g/g DW from cell suspensions which had an average taxol content o f 5 mu g/g DW pr less.