C. Klingler et al., POTENTIATION OF RECEPTOR-MEDIATED CAMP PRODUCTION - ROLE IN THE CROSS-TALK BETWEEN VASOPRESSIN V-1A AND V-2 RECEPTOR TRANSDUCTION PATHWAYS, Biochemical journal, 330, 1998, pp. 1023-1028
Cross-talk between the phospholipase C and adenylyl cyclase signalling
pathways was investigated in Chinese hamster ovary (CHO) cells transf
ected with the V-1a and V-2 vasopressin receptors. Cell lines expressi
ng V-1a, V-2, or both V-1a and V-2 receptors, were established and cha
racterized. Stimulation of V-2 receptors by vasopressin induced a dose
-dependent increase in cAMP accumulation, whereas stimulation of V-1a
receptor resulted in an increase in intracellular calcium without any
change in basal cAMP. The simultaneous stimulation of V-2 and V-1a rec
eptors by vasopressin elicited an intracellular cAMP accumulation whic
h was twice that induced by stimulation of V-2 receptor alone with dea
mino-[D-Arg(8)]vasopressin. This potentiation between V-1a and V-2 rec
eptors was mimicked by activation of protein kinase C (PKC) with PMA,
and was suppressed when PKC activity was inhibited by bisindolylmaleim
ide. The potentiation was observed in the presence or absence of 1 mM
3-isobutyl-1-methylxanthine, a phosphodiesterase inhibitor, implying t
hat an alteration in cAMP hydrolysis was not involved. Vasopressin, as
well as PMA, had no effect on the forskolin-induced cAMP accumulation
, suggesting that PKC did not directly stimulate the cyclase activity.
On the other hand, vasopressin, like PMA, potentiated the cAMP accumu
lation induced by cholera toxin, an activator of G alpha(s) protein. T
hese results suggest that, in CHO cells, vasopressin V-1a receptor pot
entiates the cAMP accumulation induced by the V-2 receptor through a P
KC-dependent increase in the coupling between Gs protein and adenylyl
cyclase.