V. Kulshrestha et Sc. Pathak, ASPERGILLOSIS IN GERMAN-COCKROACH BLATTELLA-GERMANICA (L.) (BLATTOIDEA, BLATTELLIDAE), Mycopathologia, 139(2), 1997, pp. 75-78
Natural infection of Aspergillus flavus was observed in adults of Blat
tella germanica. Though the adult insects exhibited no external sympto
ms, they became hypoactive and later died. The dead and experimentally
infected insects repeatedly yielded Aspergillus flavus in culture on
Czapek's medium. Direct microscopic observation of the tissues of infe
cted insects revealed fungal material. The blood films stained with Gi
emsa stain showed granulocytes (GRs) engulfing fungal hyphae. A remark
able increase in GR and plasmatocyte (PL) counts occurred in different
ial haemocyte counts (DHCs) of the infected insects. Two main types of
immunological responses of the insect noticed were phagocytosis and e
ncapsulation. DHC showed maximum involvement of GRs and PLs in immune
mechanism.