G. Morovjan et al., DETERMINATION OF FENBENDAZOLE, PRAZIQUANTEL AND PYRANTEL PAMOATE IN DOG PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 797(1-2), 1998, pp. 237-244
Citations number
16
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Simple and rapid high-performance liquid chromatographic methods were
developed for the determination of fenbendazole, praziquantel and pyra
ntel pamoate in dog plasma. The combination of these drugs is the most
powerful treatment against most types of worms. Blood plasma samples
obtained in a pharmacokinetic trial were prepared using solid-phase ex
traction. Fenbendazole and praziquantel were analyzed simultaneously b
y reversed-phase high-performance liquid chromatography on an octadecy
l-modified silica stationary phase employing acetonitrile-phosphate bu
ffer (pH 3.0) eluent and ultraviolet detection at 220 nm. Pyrantel was
analyzed separately on a base-deactivated reversed-phase column using
methanol-tetrahydrofuran-ammonium acetate buffer (pH 4.6) eluent and
ultraviolet detection at 317 nm. Average recoveries for fenbendazole,
praziquantel and pyrantel pamoate were 76.8, 93.4 and 90.5%, respectiv
ely. Limits of quantitation were in the range of 15-25 ng/ml plasma. (
C) 1998 Elsevier Science B.V.