HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY METHODS FOR THE SEPARATION ANDQUANTITATION OF SPIRONOLACTONE AND ITS DEGRADATION PRODUCTS IN AQUEOUS FORMULATIONS AND OF ITS METABOLITES IN RAT SERUM

HPLC assays have been developed for the determination of spironolacton e and its degradation products 7 alpha-thiospirolactone and canrenone in aqueous solutions of beta-cyclodextrins and for the determination o f spironolactone and its metabolites 7 alpha-thiospirolactone, 7 alpha -thiomethylspirolactone, 6 beta-hydroxy-7 alpha-thiomethylspirolactone and canrenone in rat serum samples. Both methods were well suited for their respective applications, i.e., studying the stability of spiron olactone in liquid formulations of beta-cyclodextrins and the oral abs orption of spironolactone in rats. The HPLC method developed for spiro nolactone and its metabolites in rat serum requires very small volumes of serum making it possible to take several blood samples over a peri od of time. (C) 1998 Elsevier Science B.V.