A HIGH-RESOLUTION METRIC HAPPY MAP OF HUMAN-CHROMOSOME-14

We have mapped 1001 novel sequence-tagged sites on human chromosome 14 . The mean spacing between markers is similar to 90 Kb, most markers a re mapped with a resolution of better than 100 kb, and physical distan ces are determined. The map was produced using HAPPY mapping, a simple and widely applicable in vitro approach that is analogous to linkage or to radiation hybrid mapping, but that circumvents many of the diffi culties and potential artifacts associated with these methods. We show also that the map serves as a robust scaffold for building physical m aps using large-insert clones. (C) 1998 Academic Press.