ISOLATION AND CHROMOSOMAL MAPPING OF THE HUMAN HOMOLOG OF PERILIPIN (PLIN), A RAT ADIPOSE TISSUE-SPECIFIC GENE, BY DIFFERENTIAL DISPLAY METHOD

Using the differential display technique, we isolated a cDNA clone enc oding the human homolog of rat perilipin, a unique protein associated with intracellular neutral lipid droplets in adipocytes and steroidoge nic cells. The full cDNA contains an open reading frame of 1566 nucleo tides encoding 522 amino acids and bears 79% amino acid identity to ra t perilipin, Northern blot analysis showed that among 20 human adult t issues examined, human perilipin was expressed specifically in adipose tissues, We determined the chromosomal location of the perilipin gene at 15q26 by means of fluorescence in situ hybridization. (C) 1998 Aca demic Press.