LIPID INTERFERENCE IN THE DETERMINATION OF THE CONCENTRATION OF HEMOGLOBIN IN PLASMA USING THE ACA SX ANALYZER

In comparison to a triple wavelength procedure, the dual wavelength me thod for the determination of plasma haemoglobin concentration using t he ACA analyzer showed considerable interference with hypertriglycerid aemic (triacylglycerols > 2.3 mmol/l) plasma. By addition of isolated human lipoprotein fractions to normotrigIyceridaemic plasma, chylomicr ons were identified as a major source of interference with the ACA pla sma haemoglobin method, whereas VLDL was without effect up to a triacy lglycerol concentration of 5.7 mmol/l. Airfuge ultracentrifugation pro ved to be a reliable means for removal of interfering lipid. We conclu de that the extent of lipid interference with the ACA plasma haemoglob in method is highly dependent on the type of lipoprotein present. An a ccurate measurement of plasma haemoglobin concentrations in non-fastin g plasma can only be ensured after lipid removal through airfuge ultra centrifugation.