The objective is to describe and quantify the diel variability of wate
r quality in a tropical coastal system, Guanabara Bay, Brazil. Water s
amples were collected in spring and neap tide cycles over 24 h periods
at three strategic sites. A pollution gradient was evident between th
e sampling sites, The average fecal coliform values decreased from 10(
6) (site 3, most polluted) to 10(1) (site 1, less polluted). Organic m
atter mineralization was found in a similar gradient to organic pollut
ion. However, complete nitrification was only found associated to regi
ons where the water quality was better. Variability in this data set w
as determined mostly by the pollution gradient observed, and by tidal
influence as well. The poor water quality indicates that the bay under
goes severe environmental stress. However water renewal promoted by ti
dal action was an important mechanism in diluting the pollution, impro
ving water quality even in ebb tides and in the inner channels. The si
gnificance of micro-scale changes in water quality assessment in Guana
bara Bay was confirmed, as well as the importance of these strategic s
ampling sites, reinforcing the importance of these measurements in mon
itoring programs.