Wcmk. Breteler et al., FEASIBILITY OF INCUBATION EXPERIMENTS TO ESTIMATE MOLTING RATES IN MARINE COPEPODS, Journal of plankton research, 20(2), 1998, pp. 257-269
Moulting rate estimates of Temora longicornis and Pseudocalanus elonga
tus in short incubation experiments in the laboratory were compared wi
th shipboard experiments in the North Sea. Absence of food, rotation o
f bottles and (an extremely small) container size did not affect the m
oulting rate in well-fed copepods. A minor bias was found due to gentl
e handling. High variance was due to the use of different samples, one
before and one after incubation, which is part of the so-called 'Kimm
erer method'. This error can be reduced by monitoring the new producti
on of exuviae in a single sample, which makes sorting of individuals u
nnecessary, while conserving the estimate of moulting rate in individu
al stages.