TIME-COURSE OF CHANGES IN THE INTESTINAL PERMEABILITY OF FOOD-SENSITIZED RATS AFTER ORAL ALLERGEN CHALLENGE

Rats were sensitized with ovalbumin (OVA) with a molecular weight of 4 5 kd, challenged with OVA orally, followed by orally administered beta -lactoglobulin (BLG) as an intestinal permeability marker. BLG is a ma cromolecular protein with a molecular weight of 18 kd. Blood BLG conce ntrations were measured (by ELISA) serially over 4 hours following BLG administration, which in turn was given 1 hour after OVA challenges. The maximum BLG concentration was at 2 hours. BLG was then administere d orally 1, 3, 6, 12 and 24 hours after oral OVA challenge, and the se rum BLG concentration at 2 hours after BLG administration was compared among the five groups. BLG appeared in the circulation of the animals 1, 6 and 24 hours after allergen challenge, but not after 3 and 12 ho urs. The serum BLG concentration was not significantly different at 1, 6 and 24 hours. Histopathological examinations of the intestines show ed mast cell infiltration of the intestinal mucosa at 1 hour, remarkab le edema of villi at 3 hours, eosinophil infiltration at 6 hours, an i ncrease of goblet cells at 12 hours and villous atrophy and lymphocyte infiltration at 24 hours. The appearance in the serum of three BLG pe aks of comparable heights suggested that the intestinal absorption of BLG may be related to a late and delayed phase as well as the immediat e IgE-dependent phase response.