CLONING OF CHICKEN AND MOUSE ALPHA(1B) ADRENERGIC-RECEPTOR

A partial cDNA encoding most of the third intracellular loop of the ch icken ct,, adrenergic receptor subtype, obtained by reverse transcript ion-polymerase chain reaction (RT-PCR) techniques using degenerate pri mers derived from mammalian sequences, was used to isolate an alpha(1b ) adrenergic receptor cDNA from brain. The cDNA encodes a potential pr otein of 507 amino acids and Northern hybridization of poly(A)(+) RNA from chicken brain of different developmental stages detected a single 3.5 kb transcript. Analysis of receptor expression indicated that the a,, adrenergic receptor is widely distributed in chicken tissues, spe cially kidney and liver. cDNA and genomic clones encoding sequences of the mouse a,, adrenergic receptor were also isolated. (C) 1998 Elsevi er Science B.V.