DETERMINATION OF BLOOD SUGARS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY WITH FLUORESCENT DETECTION

In this study, a high pressure liquid chromatography method with fluor escent detector was developed to analyze blood galactose, lactose and glucose simultaneously. Plasma sugars were prepared as fluorescent der ivatives to react with FMOC-hydrazine (9-fluorenyl methyl chloroformat e). A C-18 reversed phase column and a fluorescent detector were used and run in ambient. The resolution index of galactose and glucose deri vatives in the analytical method was 1.15. The coefficients of variati on of the analysis were less than 7.5%. The concentration of FMOC-hydr azine did not significantly influence the analytical results for deter mination of the concentration of galactose. However, the ratios of ace tonitrile in the mobile phase significantly affected the analysis of t he fluorescent derivatives of sugars. The sensitivity of this method f or galactose detection was 5 mu g ml(-1), and the required plasma volu me for testing was only 25 mu l each. This analytical method was succe ssfully applied to study the pharmacokinetics of galactose in vivo in a rabbit model. (C) 1998 Elsevier Science B.V. All rights reserved.